Major Research Interest

Human Papillomaviruses (HPVs)

Human papillomaviruses (HPVs) are the causative agents of cervical and other cancers.  As part of their normal life cycles, these small DNA viruses infect stratified epithelia and establish persistent infections, usually resulting in minor hyperproliferation, but occasionally progressing to malignancy. The goal of the laboratory is understand the evolved functions of HPV oncogenes in sustaining persistent, productive viral infection and how those functions are dysregulated during cancer development. We can model all phases of the viral life with both wild-type and genetically modified viruses using a variety of cell culture techniques, including organotypic culture, to establish persistent HPV infections in vitro.

We have discovered that the HPV oncogenes E5 and E7 regulate overlapping sets of genes that regulate the interaction between infected epithelia and cells of the stromal microenvironment, with particular impact on innate immunity. We have discovered that signals from stromal fibroblasts are critical for controlling HPV infection in the epithelium. We have shown that E5 is important for innate immune evasion and for promoting the late stages of the viral life cycle.

Selected Publications

1. JM Bodily and C Meyers.  Genetic Analysis of the Differentiation-dependent HPV31 Late Promoter. Journal of Virology, 79(6):3309-21, 2005. PMC1075705
2. S Alam, J Bromberg-White, M McLaughlin-Drubin, E Sen, JM Bodily, and C Meyers. Activity and Therapeutic Potential of ORI-1001 Antisense Oligonucleotide on Human Papillomavirus Replication Utilizing a Model of Dysplastic Human Epithelium.  Anticancer Research, 25:765-78, 2005.
3. JM Bodily, S Alam, and C Meyers.  Regulation of Human Papillomavirus Type 31 Late Promoter Activation and Genome Amplification by Protein Kinase C. Virology, 348(2):328-40, 2006.
4. JM Bodily*, M Nakamura*, M. Beglin, S Kyo, M Inoue, LA Laimins. Hypoxia-specific stabilization of HIF-1α by human papillomaviruses.  Virology, 387(2):442-8, 2009. PMC2674135  ^*Co-first authors
5. TJ McGarry, M Bonaguidi, L Lyass, JA Kessler, JM Bodily, L Doglio. Enucleation of feeder cells and egg cells with psoralens. Developmental Dynamics, 238:2614-2621, 2009 PMC2885919
6. JM Bodily, KPM Mehta, and LA Laimins.  Human papillomavirus E7 enhances hypoxia inducible factor-1 mediated transcription by inhibiting binding of histone deacetylases.  Cancer Research 71:1187-95, 2011. PMC3077548
7. JM Bodily, KPM Mehta, L Cruz, C Meyers, and LA Laimins.  The E7 Open Reading Frame Acts in Cis and Trans to Mediate Differentiation-Dependent Activities in the Human Papillomavirus Type 16 Life Cycle. Journal of Virology, 85(17):8852-62, 2011. PMC3165838
8. M Donaldson, L Mackintosh, JM Bodily, E Dornan, L Laimins, and I Morgan.  An interaction between human papillomavirus 16 E2 and TopBP1 is required for optimum viral DNA replication and episomal genome establishment. Journal of Virology, 86(23):12806-15, 2012, PMC3497701. PMC3497701
9. JM Bodily*, GA Wrobel, C Hennigan, and CM Rodriguez. Regulation of the human papillomavirus type 16 late promoter by E7 and the cell cycle. Virology 433(1):11–19, 2013 (Featured in Virology Highlights Blog). PMC5789804 *senior author
10. EJ Gauson, MM Donaldson, ES Doran, X Wang, M Bristol, JM Bodily, and IM Morgan. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2 mediated DNA replication. Journal of Virology, 1;89(9):4980-91, 2015 PMC4403487
11. E Langsfield, JM Bodily, and LA Laimins. The deacetylase Sirtuin 1 regulates human papillomavirus replication by modulating histone acetylation and recruitment of DNA damage factors NBS1 and Rad51 to viral genomes. PLoS Pathogens, 2015 Sep 25;11(9):e1005181. doi: 10.1371/journal.ppat.1005181, 2015. PMC4583417
12. T Klymenko, H Hernandez-Lopez, A MacDonald, JM Bodily, and S Graham. Human papillomavirus E2 regulates SRSF3 (SRp20) to promote capsid protein expression in infected differentiated keratinocytes. 2016 Journal of Virology 90(10):5047-5058. PMC4859725 (Spotlighted as an Article of Significant Interest)
13. SS Dykes, CF Gao, WK Songock, RL Bigelow, G Vande Woude, JM Bodily, and JA Cardelli. Zinc finger E-box binding homeobox-1 (Zeb1) drives anterograde lysosome trafficking and tumor cell invasion via upregulation of Sodium/Hydrogen Exchanger-1 (NHE1). Mol Carcinog. 2017 Feb;56(2):722-734. PMC5731239
14. WK Songock, ML Scott, and JM Bodily. Regulation of the human papillomavirus type 16 late promoter by transcriptional elongation. Virology. 2017 Apr 24;507:179-191. PMC5488730
15. M. Bienkowska-Haba, W Luszczek, JE Myers, TR Keiffer, S DiGiuseppe, P Polk, JM Bodily, RS Scott, and M Sapp. A new cell culture model to genetically dissect the complete human papillomavirus life cycle. PLoS Pathog 2018 Mar 14(3): e1006846. (Highlighted Article) PMC5833277
16. S Hong, S Cheng, W Songock, JM Bodily, and LA Laimins. Suppression of microRNA 424 levels by human papillomaviruses is necessary for differentiation-dependent genome amplification. Journal of Virology 2017 Nov 30, 91(24) e01712-17 PMC5709607

17. JT Guidry, J Meyers, M Bienkowska-Haba, WK Songock, X Ma, M Shi, CA Nathan, JM Bodily, M Sapp, R Scott. Inhibition of Epstein-Barr Virus Replication in Human Papillomavirus-Immortalized Keratinocytes. Journal of Virology 2019 93:e01216-18. https://doi.org/10.1128/JVI. PMC6321917

18. ML Scott, DT Coleman, KC Kelly, JL Carroll, BL Woodby, WK Songock, JA Cardelli, and JM Bodily. Human papillomavirus type 16 E5-mediated upregulation of Met in human keratinocytes. Virology  2018 Jun 30;519:1-11 PMC5971161

19. BL Woodby, WK Songock, ML Scott, G Raikhy, and JM Bodily. Induction of interferon kappa in human papillomavirus type 16 infection by transforming growth factor beta-induced promoter demethylation. Journal of Virology Mar 2018 92(8):e01714-17. PMC5874427
20. G Raikhy, ML Scott, BL Woodby, G Shin, JE Myers, RS Scott, and JM Bodily. Regulation of stromal gene expression by human papillomavirus type. Journal of Virology. 2019 Jul 10. pii: JVI.00458-19. doi: 10.1128/JVI.00458-19.
21. JE Myers, JT Guidry, ML Scott, K Prasai, M Bienkowska-Haba, JM Bodily, MJ Sapp, Scott RS. Detecting episomal or integrated Human Papillomavirus 16 DNA using an Exonuclease V-qPCR-based assay. Virology. 2019 Aug 22;537:149-156.
22. ML Scott, BL Woodby, J Ulicny, G Raikhy, AW Orr, WK Songock, and JM Bodily. Human papillomavirus type 16 E5 inhibits interferon signaling and supports episomal viral maintenance.  J Virol. 2020 Jan 6;94(2)
23. SM Rice, SM Kim, CM Rodriquez, WK Songock, G Raikhy, R Lopez, L Henderson, A Yusufji, and JM Bodily.  Suppression of A Subset of Interferon-Induced Genes by Human Papillomavirus Type 16 E7 via A Cyclin Dependent Kinase 8-Dependent Mechanism. Viruses 2020 Mar 13, 12:311
24. J Trammel, O Amusan, A Hultfren, G Raikhy, JM Bodily. Epidermal growth factor receptor-dependent stimulation of differentiation by human papillomavirus type 16 E5. 2023, Virology, Dec 12:590:109952.

25. JE Myers, D Schaal, E Nkadi, BJ Ward, M Bienkowska-Haba, MJ Sapp, JM Bodily, RS Scott. Retinoblastoma protein is required for Epstein-Barr virus replication in differentiated epithelia. Journal of Virology, February 2023, Volume 97, Issue 2, e01032-22

26. TC Davis, J Singh, JG Lance, L Latiolais, C Kevil, JM Bodily, M Sapp, R Scott, P Weinberger, J Vanchiere, C Arnold. COVID-19 Community Testing In Rural Areas: A Partnership between an Academic Medical Center and Community Clinics. 2020, J Community Med Health Educ 10: 686.